6MJH
The S31N mutant of the influenza A M2 proton channel in two distinct conformational states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.1158 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.290, 36.150, 76.450 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 35.236 - 2.060 |
| R-factor | 0.2199 |
| Rwork | 0.217 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lbw 5joo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.573 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.150 | 2.120 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rpim | 0.062 | 0.476 |
| Number of reflections | 11019 | 780 |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 91.1 | 82.6 |
| Redundancy | 5.7 | 5 |
| CC(1/2) | 0.997 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 277 | LCP: monoolein, M2TM S31N monomer, and 50 mM MNG-3-C8 detergent Precipitant solution: 0.2 M NaCl, 0.05 M calcium acetate pH 5.0, 29% v/v PEG 400 |
