6MGE
Structure of human 4-1BBL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9774 |
Spacegroup name | P 62 |
Unit cell lengths | 118.910, 118.910, 105.494 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.947 - 2.950 |
R-factor | 0.1971 |
Rwork | 0.194 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x29 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.612 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.950 | 3.056 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 17937 | 1796 |
<I/σ(I)> | 13.25 | 0.7 |
Completeness [%] | 99.8 | 99.05 |
Redundancy | 10.3 | 10.2 |
CC(1/2) | 0.997 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 8% w/v PEG 8000, 100mM Tris 8.5 |