6MF6

Crystal structure of budding yeast Cdc5 polo-box domain in complex with the Dbf4 polo-interacting region.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9762
Spacegroup name	P 65
Unit cell lengths	135.054, 135.054, 75.163
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.207 - 3.400
R-factor	0.2454
Rwork	0.243
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q4o
RMSD bond length	0.004
RMSD bond angle	0.711
Data reduction software	MOSFLM
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.500	3.490
High resolution limit [Å]	3.400	3.400
Number of reflections	10923
<I/σ(I)>	10.92
Completeness [%]	100.0
Redundancy	18.9
CC(1/2)	0.998	0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	200 mM sodium potassium tartrate, 10 mM trimethylamine hydrochloride, 20 % PEG 3350.