6M86
Crystal Structure of Inward Rectifier Kir2.2 Force Open Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | I 4 |
Unit cell lengths | 82.852, 82.852, 189.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.600 |
R-factor | 0.2365 |
Rwork | 0.234 |
R-free | 0.28670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3spc |
RMSD bond length | 0.004 |
RMSD bond angle | 0.851 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
Rmerge | 0.079 | 0.072 | 0.742 |
Rmeas | 0.086 | 0.079 | 0.808 |
Rpim | 0.035 | 0.032 | 0.317 |
Number of reflections | 7419 | 762 | 727 |
<I/σ(I)> | 11.1 | ||
Completeness [%] | 99.9 | 99.9 | 100 |
Redundancy | 6.7 | 6.8 | 6.4 |
CC(1/2) | 0.995 | 0.807 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293.15 | 27.4% PEG400 180 mM triSodium Citrate 100 mM TrisHCl |