6M6L
The crystal structure of glycosidase hydrolyzing Notoginsenoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.608, 95.571, 93.348 |
Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
Resolution | 35.371 - 1.500 |
R-factor | 0.151 |
Rwork | 0.150 |
R-free | 0.17410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ta9 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.960 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.490 | 1.490 |
Number of reflections | 147102 | 147102 |
<I/σ(I)> | 29.5 | |
Completeness [%] | 96.9 | |
Redundancy | 3.8 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.1 M Sodium citrate tribasic dehydrate (pH 5.6), 0.2 M ammonium acetate and PEG 4000 (30%, w/v) |