6M2D
MUL1-RING domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2013-06-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.2837 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.226, 66.593, 68.271 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.671 - 1.795 |
R-factor | 0.1744 |
Rwork | 0.172 |
R-free | 0.22720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t6p |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 10.000 |
High resolution limit [Å] | 1.795 | 1.800 |
Rmerge | 0.080 | |
Rpim | 0.026 | 0.026 |
Number of reflections | 25501 | 25501 |
<I/σ(I)> | 24.5 | |
Completeness [%] | 96.8 | |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.4 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3350 |