6LVH
Crystal structure of methionine aminopeptidase from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS IV |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-10-29 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 62 |
Unit cell lengths | 133.658, 133.658, 62.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.450 - 3.200 |
R-factor | 0.1646 |
Rwork | 0.162 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wkm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.740 |
Data reduction software | DENZO (2.3.1) |
Data scaling software | SCALEPACK (2.2.0) |
Phasing software | MOLREP (11.7.01) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.850 | 3.200 |
Rmerge | 0.073 | 0.036 | 0.398 |
Rmeas | 0.086 | 0.043 | 0.496 |
Rpim | 0.045 | 0.022 | 0.291 |
Total number of observations | 36517 | ||
Number of reflections | 10354 | 1103 | 896 |
<I/σ(I)> | 10.8 | ||
Completeness [%] | 97.0 | 98.8 | 86 |
Redundancy | 3.5 | 3.7 | 2.5 |
CC(1/2) | 0.998 | 0.823 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1 M Sodium acetate, 2M NaCl, 5% glycerol |