6LS8
The monomeric structure of G80A/H81A/H82A myoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-22 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 59.147, 61.490, 70.323 |
Unit cell angles | 78.83, 81.14, 63.12 |
Refinement procedure
Resolution | 43.409 - 2.300 |
R-factor | 0.2188 |
Rwork | 0.216 |
R-free | 0.27390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wla |
RMSD bond length | 0.011 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.078 | 0.594 |
Number of reflections | 37657 | 1876 |
<I/σ(I)> | 10.1 | |
Completeness [%] | 98.0 | |
Redundancy | 1.9 | |
CC(1/2) | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M Tris-HCl buffer, 15% (w/v) PEG 6000 |