6LPE
Phascolosoma esculenta ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 232.760, 232.760, 232.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.180 - 1.990 |
| R-factor | 0.252 |
| Rwork | 0.249 |
| R-free | 0.30160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ajo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.541 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 164.590 | 2.030 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 79720 | 3603 |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 2 | |
| CC(1/2) | 0.993 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 0.1 M MES monohydrate pH 6.5, 1.6 M magnesium sulfate heptahydrate |
