6LJO

African swine fever virus dUTPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	I 21 3
Unit cell lengths	98.014, 98.014, 98.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.060 - 2.280
R-factor	0.2307
Rwork	0.228
R-free	0.27350
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.577
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.073	0.534
Rmeas	0.087	0.695
Rpim	0.014	0.682
Number of reflections	7330	371
<I/σ(I)>	1.74	2.14
Completeness [%]	100.0	100
Redundancy	3.87	3.69
CC(1/2)	0.997	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	0.5M NaH2PO4/K2HPO4,PH 6.9