6LFD
Crystal structure of VMB-1 at pH5.5(Bis-Tris)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.110, 97.110, 132.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.690 - 1.920 |
R-factor | 0.2023 |
Rwork | 0.200 |
R-free | 0.23910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jv4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.452 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.690 | 1.970 |
High resolution limit [Å] | 1.920 | 1.920 |
Number of reflections | 72855 | 5313 |
<I/σ(I)> | 4.2 | 2.1 |
Completeness [%] | 100.0 | |
Redundancy | 13.1 | |
CC(1/2) | 0.913 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3350 |