6LC9
Crystal structure of AmpC Ent385 complex form with ceftazidime
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.389, 74.083, 102.822 |
Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
Resolution | 43.670 - 1.650 |
R-factor | 0.1642 |
Rwork | 0.163 |
R-free | 0.19420 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 0.928 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.700 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.057 | 0.773 |
Number of reflections | 89346 | 14132 |
<I/σ(I)> | 18.84 | |
Completeness [%] | 98.1 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 1.8 M ammonium sulfate, 0.1 M MES pH 5.5 and 5% 1,4-Dioxane |