6LC7
Crystal structure of AmpC Ent385 free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.601, 75.204, 103.411 |
Unit cell angles | 90.00, 95.10, 90.00 |
Refinement procedure
Resolution | 46.900 - 1.400 |
Rwork | 0.147 |
R-free | 0.16800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xhr |
RMSD bond length | 0.004 |
RMSD bond angle | 0.762 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.900 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.049 | 0.660 |
Number of reflections | 149918 | 23824 |
<I/σ(I)> | 18.66 | |
Completeness [%] | 98.7 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 1.8 M ammonium sulfate, 0.1 M MES pH 5.5 and 5% 1,4-Dioxane |