6LB5
Crystal structure of dimeric RXR-LBD complexed with full agonist NEt-3IB and TIF2 co-activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.411, 64.026, 46.631 |
Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
Resolution | 46.580 - 2.400 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lb4 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.601 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 20449 | 3277 |
<I/σ(I)> | 7.4 | |
Completeness [%] | 99.3 | 99.2 |
Redundancy | 3.26 | 3.33 |
CC(1/2) | 0.996 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Imidazole 20 % PEG 6000 |