6L6L
Structural basis of NR4A2 homodimers binding to selective Nur-responsive elements
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.405, 64.315, 60.776 |
Unit cell angles | 90.00, 96.84, 90.00 |
Refinement procedure
Resolution | 35.226 - 2.781 |
R-factor | 0.2199 |
Rwork | 0.216 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cit |
RMSD bond length | 0.004 |
RMSD bond angle | 0.751 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.230 | 2.880 |
High resolution limit [Å] | 2.781 | 2.781 |
Rmerge | 0.116 | 0.374 |
Rmeas | 0.138 | |
Number of reflections | 8163 | 737 |
<I/σ(I)> | 6.69 | 2.01 |
Completeness [%] | 98.7 | 89.99 |
Redundancy | 3.3 | 3 |
CC(1/2) | 0.993 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 50 mM Na acetate (pH 4.7), 200 mM NaCl, 10 mM MgCl2, 5 mM Li2SO4, 5 mM DTT, and 15-19% PEG4K (w/v) |