6L24
Crystal structure of CK2a1 H115Y/V116I with hematein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-03-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.588, 78.823, 79.964 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.350 - 2.400 |
R-factor | 0.191778227782 |
Rwork | 0.184 |
R-free | 0.26299 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3war |
RMSD bond length | 0.008 |
RMSD bond angle | 1.242 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.350 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.193 | 0.845 |
Number of reflections | 13323 | 1373 |
<I/σ(I)> | 8 | |
Completeness [%] | 100.0 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Ethylene glycol |