6L07
Crystal structure of Escherichia coli phosphatidylserine decarboxylase (PE-bound form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 218.191, 218.191, 143.798 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.244 - 3.600 |
R-factor | 0.2434 |
Rwork | 0.241 |
R-free | 0.28910 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.820 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmeas | 0.084 | 1.136 |
Number of reflections | 45175 | 14239 |
<I/σ(I)> | 12.3 | 1.47 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 5.38 | 5.19 |
CC(1/2) | 0.999 | 0.598 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | pentaerythritol ethoxylate, ammonium sulfate |