6L06
Crystal structure of Escherichia coli phosphatidylserine decarboxylase (apo-form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 156.882, 172.012, 80.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.060 - 2.600 |
R-factor | 0.2332 |
Rwork | 0.231 |
R-free | 0.27570 |
Structure solution method | SAD |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmeas | 0.077 | 1.009 |
Number of reflections | 67495 | 20374 |
<I/σ(I)> | 11.35 | 1.03 |
Completeness [%] | 99.5 | 97.6 |
Redundancy | 3.58 | 3.6 |
CC(1/2) | 0.998 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Tacsimate |