6KXW

Crystal structure of human aquaporin AQP7 in bound to glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97775
Spacegroup name	P 21 21 21
Unit cell lengths	93.508, 106.286, 214.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.614 - 3.700
R-factor	0.2707
Rwork	0.268
R-free	0.32210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f7h
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.614	3.833
High resolution limit [Å]	3.700	3.700
Number of reflections	16925	311
<I/σ(I)>	9.05
Completeness [%]	99.9
Redundancy	20
CC(1/2)	0.973	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	30% v/v PEG 400, 50mM CAPS, pH 10.0, 0.16% w/v 1,5-Naphthalenedisulfonic acid disodium, 0.16% w/v Naphthalene-1,3,6-trisulfonic acid trisodium, 0.16% 1,4-piperazinediethanesulfonic acid