6KX2
Crystal structure of GDP bound RhoA protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.819, 91.819, 56.861 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.342 - 1.454 |
R-factor | 0.1855 |
Rwork | 0.184 |
R-free | 0.21040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftn |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.342 | 1.506 |
High resolution limit [Å] | 1.454 | 1.454 |
Rmerge | 0.113 | 0.636 |
Number of reflections | 43269 | 4263 |
<I/σ(I)> | 22.97 | |
Completeness [%] | 100.0 | |
Redundancy | 26 | |
CC(1/2) | 0.997 | 0.944 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 10 mg/mL mutant, 0.2M NaCl; 0.1M acetate Na (pH 4.5) and 1.26M ammonium sulfate |