6KVR
Fatty acid amide hydrolase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-02 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9897 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.874, 68.753, 100.979 |
Unit cell angles | 90.00, 99.63, 90.00 |
Refinement procedure
Resolution | 28.110 - 2.200 |
R-factor | 0.1726 |
Rwork | 0.171 |
R-free | 0.21760 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.925 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 58215 | 5814 |
<I/σ(I)> | 32.4 | |
Completeness [%] | 99.8 | |
Redundancy | 7 | |
CC(1/2) | 0.900 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 280 | 100mM Tris/HCl (pH 8.5), 200mM Lithium sulfate, 30% w/v PEG 4000 |