6KUI
Active conformation of HslV from Staphylococcus aureus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97960 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 95.670, 108.776, 123.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.250 - 2.330 |
R-factor | 0.2004 |
Rwork | 0.196 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kr1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.460 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmeas | 0.073 | 0.400 |
Rpim | 0.021 | 0.184 |
Number of reflections | 27546 | 1245 |
<I/σ(I)> | 24.5 | 2.5 |
Completeness [%] | 98.6 | |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | Bis-Tris propane, Citric acid, PEG 3350 |