6KTX
The wildtype structure of EanB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.583, 111.387, 60.285 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.149 - 2.189 |
Rwork | 0.172 |
R-free | 0.21330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ipp |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.210 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 30763 | 1457 |
<I/σ(I)> | 9.5 | |
Completeness [%] | 99.6 | 95.2 |
Redundancy | 9.4 | |
CC(1/2) | 0.971 | 0.677 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.02M MgCl2, 0.1M Hepes 7.5, 22%w/v polyacrylic acid sodium salt 5100 |