6KOG
Ketosynthase domain in tenuazonic acid synthetase 1 (TAS1).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 103 |
Detector technology | PIXEL |
Collection date | 2017-10-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.633, 99.136, 127.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.568 - 1.680 |
R-factor | 0.166 |
Rwork | 0.165 |
R-free | 0.19240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mz0 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.323 |
Data reduction software | XDS |
Data scaling software | Aimless (1.11.4) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.568 | 1.670 |
High resolution limit [Å] | 1.575 | 1.580 |
Number of reflections | 129538 | 87079 |
<I/σ(I)> | 8.61 | |
Completeness [%] | 80.6 | 35 |
Redundancy | 6.8 | |
CC(1/2) | 0.998 | 0.663 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 120 mM NaCl, 1.65 M Ammonium sulfate, 0.1 M MES-NaOH pH 6.5, 5% Methanol |