6KMU
P22/P10 complex of caspase-11 mutant C254A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2018-10-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97892 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.971, 102.002, 99.744 |
Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
Resolution | 49.780 - 2.100 |
R-factor | 0.2109 |
Rwork | 0.210 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kmt |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.780 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.057 | 0.369 |
Number of reflections | 71527 | 5274 |
<I/σ(I)> | 11.81 | 2.72 |
Completeness [%] | 97.7 | |
Redundancy | 3.41 | |
CC(1/2) | 0.997 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES (pH 7.5), 12% (w/v) polyethylene glycol 3350, and 4% formaldehyde |