6KMR
Crystal structure of human N6amt1-Trm112 in complex with SAM (space group P6122)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 109.708, 109.708, 130.786 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.026 - 2.000 |
| R-factor | 0.1632 |
| Rwork | 0.162 |
| R-free | 0.19180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kms |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.128 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.115 | 0.073 | 0.608 |
| Rmeas | 0.125 | 0.080 | 0.661 |
| Rpim | 0.049 | 0.031 | 0.254 |
| Total number of observations | 206280 | ||
| Number of reflections | 31971 | 3454 | 3126 |
| <I/σ(I)> | 4.3 | ||
| Completeness [%] | 99.7 | 99.3 | 99.6 |
| Redundancy | 6.5 | 6.2 | 6.5 |
| CC(1/2) | 0.993 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M succinic acid, pH 7.0, 15% PEG 3350 |
