6KBE
Structure of Deubiquitinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.303, 58.763, 110.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.654 - 2.339 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zfy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.12_2829: V1.0)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmeas | 0.085 | 0.447 |
| Number of reflections | 98958 | 4887 |
| <I/σ(I)> | 4.12 | |
| Completeness [%] | 96.6 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 0.2 M Sodium chloride, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350 |
