6K7O
Complex structure of LILRB4 and h128-3 antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0401 |
Spacegroup name | P 62 |
Unit cell lengths | 187.276, 187.276, 183.819 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.214 - 3.004 |
R-factor | 0.2302 |
Rwork | 0.228 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p2t |
RMSD bond length | 0.004 |
RMSD bond angle | 0.660 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 72345 | 7232 |
<I/σ(I)> | 15.971 | 2.595 |
Completeness [%] | 99.7 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M ammonium dihydrogen phosphate, 0.1M Tris pH8.5, 50 % v/v MPD |