6K2S

Crystal structure of proteinase K from Engyodontium album

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	300
Detector technology	CCD
Collection date	2019-01-30
Detector	MPCCD
Wavelength(s)	1.24
Spacegroup name	P 43 21 2
Unit cell lengths	68.280, 68.280, 108.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.500 - 1.600
R-factor	0.1632
Rwork	0.162
R-free	0.18630
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.009
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.500	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	34574	1700
<I/σ(I)>	7.3
Completeness [%]	100.0
Redundancy	265

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.5	293	0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES