6JIA
Marine bacterial laminarinase mutant E135A complex with laminaritetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-11-17 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.863, 95.456, 72.381 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.880 - 1.900 |
R-factor | 0.1521 |
Rwork | 0.150 |
R-free | 0.18670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4crq |
RMSD bond length | 0.012 |
RMSD bond angle | 1.689 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.730 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.067 | 0.460 |
Number of reflections | 18576 | 1147 |
<I/σ(I)> | 17.5 | 3.5 |
Completeness [%] | 99.8 | 97.4 |
Redundancy | 6.3 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5% (v/v) ethonal, 28% PEG20000 100 mM MES pH7.0 |