6JEH
Crystal structure of calcium free human gelsolin amyloid mutant D187Y
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2014-10-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 169.232, 169.232, 151.087 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.944 - 2.950 |
R-factor | 0.1962 |
Rwork | 0.193 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ffn |
RMSD bond length | 0.011 |
RMSD bond angle | 1.367 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.944 | 3.050 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 46032 | |
<I/σ(I)> | 1.87 | |
Completeness [%] | 98.9 | |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl pH8.5 |