6J9O

Crystal structure of a free scFv molecule from a group 2 influenza A viruses HA binding antibody AF4H1K1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	36.747, 56.798, 102.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.594 - 1.397
R-factor	0.1771
Rwork	0.176
R-free	0.19170
RMSD bond length	0.003
RMSD bond angle	0.673
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.430
High resolution limit [Å]	1.397	1.400
Number of reflections	42860
<I/σ(I)>	39.3
Completeness [%]	98.7
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1.4 M sodium malonate, 0.1 M Bis-Tris propane, pH 7.0