6J7O
Crystal structure of toxin TglT (unusual type guanylyltransferase-like toxin, Rv1045) mutant E146Q from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.443, 95.443, 69.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.280 - 1.900 |
R-factor | 0.1963 |
Rwork | 0.195 |
R-free | 0.21620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j7t |
RMSD bond length | 0.007 |
RMSD bond angle | 0.924 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.280 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.078 | 1.120 |
Number of reflections | 28982 | 4601 |
<I/σ(I)> | 17.42 | 2.01 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 9.97 | 9.94 |
CC(1/2) | 0.999 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.2M magnesium chloride hexahydrate;0.1M bis-tris ph6.5;25%PEG3350 Cryo:10%ethylene glycol |