Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.039, 54.446, 66.070 |
Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.201 |
R-factor | 0.1343 |
Rwork | 0.133 |
R-free | 0.15680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.007 |
RMSD bond angle | 0.931 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.047 | 0.156 |
Number of reflections | 70798 | 3461 |
<I/σ(I)> | 28.2 | 11.6 |
Completeness [%] | 98.5 | 96.5 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 38% PEG 400, 0.1 M Tris pH 8.0 |