6IQK
crystal structure of Arabidopsis thaliana Profilin 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2017-12-03 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.900, 153.370, 196.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.240 - 3.600 |
| R-factor | 0.3128 |
| Rwork | 0.309 |
| R-free | 0.34650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.549 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 3.729 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 48279 | 48279 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 15 | |
| CC(1/2) | 0.991 | 0.275 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.1M Tris HCl (pH 7.8), 0.9M sodium citrate |
