6IQF

crystal structure of Arabidopsis thaliana Profilin 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.954
Spacegroup name	C 1 2 1
Unit cell lengths	80.987, 52.862, 32.410
Unit cell angles	90.00, 110.38, 90.00

Refinement procedure

Resolution	43.381 - 1.457
R-factor	0.193
Rwork	0.192
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nul
RMSD bond length	0.006
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.400	1.509
High resolution limit [Å]	1.457	1.457
Number of reflections	44096	6286
<I/σ(I)>	8.77
Completeness [%]	99.0
Redundancy	3.26
CC(1/2)	0.996	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	0.1M sodium acetate (pH 5.0), 1.42M ammonium sulfate