6IEP
GAPDH of Streptococcus agalactiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.580, 124.420, 125.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k73 |
RMSD bond length | 0.007 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.170 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.157 | 0.430 |
Rmeas | 0.170 | 0.475 |
Rpim | 0.049 | 0.152 |
Number of reflections | 43229 | |
<I/σ(I)> | 13 | 5.7 |
Completeness [%] | 99.9 | |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 40% PEG 4000 monomethyl ether and 100 mM Tris-HCl pH 9.0 |