6IEC
Structure of RVFV Gn and human monoclonal antibody R17
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 62 |
Unit cell lengths | 198.009, 198.009, 164.467 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.560 - 3.200 |
R-factor | 0.2428 |
Rwork | 0.241 |
R-free | 0.28080 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.707 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rpim | 0.090 | 0.030 |
Number of reflections | 58821 | |
<I/σ(I)> | 7.3 | 2 |
Completeness [%] | 97.2 | |
Redundancy | 6.5 | |
CC(1/2) | 0.986 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2M ammonium sulfate, 0.1M Tris 7.5, 20% w/v PEG 5000 MME |