6ID9

Crystal structure of H7 hemagglutinin mutant H7-SGTL ( A138S, V186G, P221T) from the influenza virus A/Anhui/1/2013 (H7N9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97923
Spacegroup name	H 3 2
Unit cell lengths	116.268, 116.268, 293.839
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.528 - 2.700
R-factor	0.2471
Rwork	0.245
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kol
RMSD bond length	0.002
RMSD bond angle	0.600
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	20977
<I/σ(I)>	13.24
Completeness [%]	97.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.0M Ammonium sulfate, 0.1M Sodium HEPES pH 7.5, 5% v/v PEG 400