6I3P
Crystal structure of DEAH-box ATPase Prp22 with bound ssRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 139.790, 140.500, 159.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.240 - 2.750 |
R-factor | 0.2233 |
Rwork | 0.222 |
R-free | 0.25310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fa5 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.772 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.240 | 50.000 | 2.870 |
High resolution limit [Å] | 2.750 | 49.240 | 2.750 |
Rmerge | 0.110 | 1.469 | |
Rmeas | 0.118 | 1.567 | |
Total number of observations | 639883 | ||
Number of reflections | 82130 | 9753 | |
<I/σ(I)> | 14.28 | 1.51 | |
Completeness [%] | 99.9 | 100 | |
Redundancy | 7.791 | 8.154 | |
CC(1/2) | 0.999 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100mM Bis-Tris propane pH8.5, 250mM NaF, 22% PEG3350 |