6HL9
Crystal Structure of the CsiD Glutarate Hydroxylase in complex with Succinate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 123.034, 123.034, 138.327 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.109 - 2.300 |
R-factor | 0.1869 |
Rwork | 0.186 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gpe |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.109 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 47789 | |
<I/σ(I)> | 22.43 | |
Completeness [%] | 98.3 | |
Redundancy | 26.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 1.0 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 |