6HKG
Structure of FISW84 Fab Fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 130.160, 130.160, 167.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.749 - 1.870 |
R-factor | 0.1835 |
Rwork | 0.183 |
R-free | 0.19950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vxs 2vxv |
RMSD bond length | 0.007 |
RMSD bond angle | 0.902 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.750 | 1.920 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmeas | 0.086 | |
Number of reflections | 118143 | 8595 |
<I/σ(I)> | 11.5 | 1 |
Completeness [%] | 99.6 | |
Redundancy | 5.9 | 5.7 |
CC(1/2) | 0.998 | 0.288 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 298 | 2.04 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 Cryoprotected in 2.1 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 and 25 % (v/v) ethylene glycol |