6H5U
MamM CTD H264E - Zinc form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97624 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.049, 55.648, 63.767 |
Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
Resolution | 47.030 - 2.000 |
R-factor | 0.17238 |
Rwork | 0.170 |
R-free | 0.22053 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w5x |
RMSD bond length | 0.014 |
RMSD bond angle | 1.467 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.030 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | |
Rmeas | 0.087 | |
Rpim | 0.025 | |
Number of reflections | 20715 | |
<I/σ(I)> | 23.2 | 13.9 |
Completeness [%] | 99.8 | 96.3 |
Redundancy | 11.8 | |
CC(1/2) | 0.997 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium sulfate, 0.1M BIS-TRIS pH=6.5, 25% PEG 3350, 1.7 mM ZnCl2 |