6H3O
Alcohol oxidase from Phanerochaete chrysosporium mutant F101S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-08 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.9726 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 112.074, 203.553, 116.009 |
Unit cell angles | 90.00, 104.64, 90.00 |
Refinement procedure
Resolution | 49.190 - 2.500 |
R-factor | 0.21387 |
Rwork | 0.210 |
R-free | 0.28560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h3g |
RMSD bond length | 0.006 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.200 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.657 | |
Rpim | 0.650 | |
Number of reflections | 170450 | 8298 |
<I/σ(I)> | 6.3 | 1.4 |
Completeness [%] | 98.5 | 96.8 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.979 | 0.485 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 14% (w/v) PEG3350 and 0.2 M potassium acetate |