6GRB

eukaryotic junction-resolving enzyme GEN-1 binding with Potassium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-12-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.975
Spacegroup name	P 31 2 1
Unit cell lengths	97.960, 97.960, 119.761
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.227 - 2.400
R-factor	0.2479
Rwork	0.246
R-free	0.27280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cnq
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.840	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	26441
<I/σ(I)>	5.8
Completeness [%]	99.8
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		280	HEPES pH 7.0 20% PEG 10000