6GPD
Crystal structure of the ligand-free form of domain 1 from TmArgBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-26 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 35.445, 46.036, 52.496 |
Unit cell angles | 116.01, 109.85, 90.01 |
Refinement procedure
Resolution | 14.400 - 1.750 |
R-factor | 0.16502 |
Rwork | 0.164 |
R-free | 0.18833 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4prs |
RMSD bond length | 0.021 |
RMSD bond angle | 1.950 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.044 | |
Number of reflections | 24812 | |
<I/σ(I)> | 37.2 | |
Completeness [%] | 88.8 | |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | The best crystals of D1 domain were obtained using a protein concentration of 6 mg/ml in a solution containing 0.2 M NaCl, 0.1M Bis-Tris (pH 5.5), 25% (w/v) polyethylene glycol (PEG) 3,350. |