6GFM
Crystal structure of the Escherichia coli nucleosidase PpnN (pppGpp-form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97623 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 176.920, 176.920, 94.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.700 - 2.770 |
Rwork | 0.181 |
R-free | 0.23190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gfl |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.700 | 2.869 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.142 | 2.704 |
Rmeas | 0.145 | 2.781 |
Rpim | 0.029 | 0.642 |
Number of reflections | 19332 | 1907 |
<I/σ(I)> | 17.45 | 1.35 |
Completeness [%] | 99.8 | 99.74 |
Redundancy | 24.8 | 18.2 |
CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277.15 | 0.4M KNa tartrate hydrate |