6GCO
Truncated FtsH from A. aeolicus in P312
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-25 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000000 |
Spacegroup name | P 3 1 2 |
Unit cell lengths | 117.304, 117.304, 137.054 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 81.613 - 3.323 |
R-factor | 0.2491 |
Rwork | 0.240 |
R-free | 0.26540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 4ww0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.649 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 81.613 | 81.613 | 3.520 |
High resolution limit [Å] | 3.320 | 9.900 | 3.320 |
Rmerge | 0.099 | 0.025 | 1.071 |
Rmeas | 0.104 | 0.027 | 1.131 |
Number of reflections | 15949 | 656 | 2390 |
<I/σ(I)> | 18.53 | 79.49 | 2.18 |
Completeness [%] | 98.8 | 99.5 | 92.5 |
Redundancy | 10.04 | 9.02 | 9.469 |
CC(1/2) | 0.999 | 0.999 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 298 | 0.2 M KCl, 0.05 M HEPES/NaOH pH7.75, 33% pentaerythritol propoxylate 426 The affinity tag was cleaved by thrombin. This cleavage was just partial according to a SDS-PAGE analysis. |