6GAV

Extremely 'open' clamp structure of DNA gyrase: role of the Corynebacteriales GyrB specific insert

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	97.506, 157.617, 103.504
Unit cell angles	90.00, 98.11, 90.00

Refinement procedure

Resolution	46.150 - 2.600
R-factor	0.199
Rwork	0.197
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3IFZ 3IG0 3ZKD
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.150
High resolution limit [Å]	2.600	2.600
Rmerge	0.104	1.253
Number of reflections	94824
<I/σ(I)>	8.9
Completeness [%]	99.2
Redundancy	3.38
CC(1/2)	0.995	0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	100 mM Sodium Acetate 100 mM MES pH 6.5 26% PEG 400 25% EG 10 mM MgCl2