6G8T
Crystal Structures of the Single PDZ Domains from GRASP65 and their Interaction with the Golgin GM130
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.377, 63.163, 80.805 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.930 - 2.670 |
| R-factor | 0.21138 |
| Rwork | 0.209 |
| R-free | 0.25794 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rle |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.251 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.930 | 2.740 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmeas | 0.062 | 0.750 |
| Number of reflections | 3573 | 260 |
| <I/σ(I)> | 20.67 | 2.28 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.3 | 4.4 |
| CC(1/2) | 0.990 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 24% PEG1500, 20% glycerol |
